Stand or crouch near the cat and let him or her approach you. When the cat comes close, let him or her sniff your hand. If the cat seems nervous, you may have to come back later or have a partner help you with the next step.
If the cat doesn’t mind being handled, this may be easiest to perform while sitting on a chair or the couch, so you don’t have to worry about dropping the cat. If you’re working with a partner, have the partner hold the cat securely in both arms while you lift the tail. If the cat avoids having his or her tail lifted, try scratching him or her in the area where the tail meets the back. Cats usually lift their tails when they are touched in this spot. [1] X Research source
A male cat will have an anus, scrotum, and penis, while a female cat will have only an anus and urinary tract opening. In an entire (or tom) cat, the scrotum is covered with fur and contains two testicles, each typically ranging in size from the size of cherry pits to actual cherries. The scrotum sticks out from the male cat’s rear as a fairly obvious pair of bumps. If the cat has long fur, this can make the scrotum more difficult to see, in which case try dampening the fur with water to flatten it down and make the scrotum more obvious. A neutered male cat will still have a scrotum, though it will typically be smaller. [2] X Research source The penis is sited below the scrotum, beneath the skin, and exits at a small furry mound between the cat’s thighs. You might imagine a male cat’s genitalia resembling a colon (:). A male cat’s anus and urinary tract opening are at least 1 inch (2. 5 cm) apart, or 1⁄2 inch (1. 3 cm) for kittens.
A female cat will have an anus and a urinary tract opening/vulva, with the vulva in the shape of a vertical slit. You can imagine a female cat’s genitalia as resembling a semi-colon (;). [3] X Research source A female cat will have a shorter distance between anus and vulva, typically about 1⁄2 inch (1. 3 cm) apart.
Cats that are calico or tortoiseshell colored are typically female. More orange or ginger colored cats are male than female, but this is not an accurate way to determine a cat’s sex. [4] X Trustworthy Source Washington State University College of Veterinary Medicine Leading veterinary medicine training institution and biomedical research center Go to source
Male cats that have not been neutered tend to be more aggressive than females and to have larger heads and thick skin. They like to roam, sometimes leaving for a few days at a time. They mark their territory by spraying it with strong-smelling urine. [5] X Research source Female cats are less likely to spray their surroundings.
Making vocal sounds to attract males. The cat may sound like it is in pain or whining. Moving the tail to the side to show genitalia or crouching into a receptive posture. The vulva may be secreting a clear discharge. Rubbing up against inanimate objects, owners, or other animals more than usual. [6] X Research source Pregnant female cats have low-hanging, distended bellies. Female cats that have given birth may have nipples that protrude from their bellies. Be careful when using nipples to determine sex, since both male and female cats have nipples.