You won’t need more than 5 mLs of water. Fill the beaker with the water. Make sure the pipette tips are the correct tips that properly fit the pipette.

Knowing the temperature of the water is important for the calculations that will be done to check the calibration.

Zeroing the scale subtracts the weight of the plastic boat and allows you to measure just the weight of whatever you put into the boat.

For calibration, test both the smallest volume and the largest volume the pipette can dispense.

Push the button to the second stop to dispense any liquid that remains in the tip and remove the pipette from the water.

Make sure the tip is completely immersed during the whole aspiration process. You don’t want any bubbles in the tip or the results will be wrong.

Keep the tip on the pipette as you will use it again to weigh out more calibration tests. MAke sure the tip does not touch anything.

It’s important to wait until the number has stabilized before recording it. Your readings will be incorrect if you don’t wait.

You can repeat this same process with different volumes as long as you take multiple readings per volume.

You can find the Z variable by looking up the density of water using the temperature recorded at the beginning of the experiment. [10] X Research source For example: If the temperature of the water was 23°C, then you will use a Z value of 1. 0035 µg/mg.

For example: The ten weights you recorded for a pipette volume of 10µL are as follows: 9. 89, 10. 01, 10. 02, 9. 99, 9. 95, 10. 04, 9. 96, 10. 01, 9. 99, and 9. 98. The average is: (9. 89 + 10. 01 + 10. 02 + 9. 99 + 9. 95+ 10. 04 + 9. 96 + 10. 01 + 9. 99 + 9. 98)/10 = 99. 84/10 = 9. 984

For example: V = w * Z = 9. 984 * 1. 0035 = 10. 019

If the pipette is properly calibrated, the calculated value should be very close to the actual value you set on the pipette. For example: A = 100 x Vavg/V0 = 100 x 10. 019/10 = 100 x 1. 0019 = 100. 19% This particular pipette is properly calibrated.

Contact the company that makes your particular brand of pipette for servicing.